Lack of Association Between CCR3 rs6441961 T>C Polymorphism and Celiac Disease in Punjabi Pakistani Population

Abstract

BACKGROUND

Celiac disease (CD) is an autoimmune disease characterized by gluten intolerance. CCR3 rs6441961 T>C polymorphism is widely studied in European populations, while Asian populations are the least studied for the rs6441961-associated CD risk.

METHODS 

To achieve the objectives of the current study, a tetra-primer amplification refractory mutation system polymerase chain reaction (tetra-ARMS-PCR) genotyping assay was developed for genotyping of CCR3 rs6441961 T>C polymorphism.

RESULTS

The current study explored the association of rs6441961 with CD in the Punjabi Pakistani population and revealed that the TT and TC genotypes were prevalent in healthy controls and the patient group, while the CC genotype was only predominant in the patient group (25.8% vs. 18.2%; χ2=0.74, P=0.69).

CONCLUSION

Multinomial regression analysis and Chi-square test indicate that the risk of CD in the Punjabi Pakistani population is not influenced by CCR3 rs6441961 T>C polymorphism.