An accessible and Pragmatic Experimental Model of Nonalcoholic Fatty

Hadi Emamat, Maryam Noori, Forough Foroughi, Marjan Rismanchi, Hassan Eini–Zinab, Azita Hekmatdoost



There is no convenient cheap pragmatic experimental model for Nonalcoholic Fatty Liver Disease (NAFLD)/Nonalcoholic Steatohepatitis (NASH).

We aimed to create a pragmatic model of NAFLD/NASH.


Sprague-Dawley rats were fed a high-fat, high sugar homemade diet ad libitum for seven weeks. The high-fat, high sugar diet included 59% of energy derived from fat, 30% from carbohydrates, and 11% from protein. Serum levels of fasting glucose, triglyceride, cholesterol, liver enzymes, insulin, and hepatic tumor necrosis factor-alpha (TNF-α) gene expression were determined. Hepatic histology was examined by H&E stain.


Rats fed the high-fat, high sugar diet developed hepatic steatosis, and a moderate inflammation, which was associated with increased serum levels of liver enzymes, glucose, insulin, triglyceride, cholesterol, and hepatic  TNF-α gene expression.


This rat model resembles the key features of human NAFLD/NASH and provides a simple pragmatic experimental model for elucidating the disease prevention and treatment.



Experimental model; NAFLD; NASH; Rat; Nonalcoholic Fatty Liver Disease; Diet

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